| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
Met1-Thr239 |
| similar : |
CD274 |
| Alias : |
CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
| Conjugate : |
C-hFc |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bioactivity : |
1. Immobilized human PD-1 at 2 μg/ml (100 μl/well) can bind human B7-H1, the EC50 of human B7-H1 is 150-600 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag) bound to Atezolizuma was 0.5 nM. |
| MolecularMass : |
The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| GeneName : |
CD274 CD274 molecule [ Homo sapiens ] |
| GeneNameWeblink : |
29126 |
| OfficialSymbol : |
CD274 |
| OfficialSymbolWeblink : |
29126 |
| GeneID : |
29126 |
| GeneIDWeblink : |
29126 |
| mRNARefseq : |
NM_014143 |
| mRNARefseqWeblink : |
NM_014143.3 |
| ProteinRefseqWeblink : |
NP_054862.1 |
| MIMWeblink : |
605402 |
| UniProtID : |
Q9NZQ7 |
| UniProtIDWeblink : |
Q9NZQ7 |
