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Recombinant Human Mediator Complex Subunit 4, His-tagged

Cat. No.: MED4-2406H
Product Overview: Recombinant human MED4, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 30.7kDa (278aa).
Description: Mediator complex subunit 4(MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36). This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner.
Source: E.coli.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol,1mM DTT, 100mM NaCl.
Purity: > 80% by SDS – PAGE.
Concentration: 0.5 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: MED4 mediator complex subunit 4 [ Homo sapiens ]
Synonyms: mediator complex subunit 4; VDRIP; DRIP36; TRAP36; HSPC126; FLJ10956; RP11-90M2.2; MED4; mediator of RNA polymerase II transcription subunit 4; ARC36; p36 TRAP/SMCC/PC2 subunit; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D receptor interacting protein; activator-recruited cofactor 36 kDa component; mediator of RNA polymerase II transcription, subunit 4 homolog; vitamin D3 receptor-interacting protein complex 36 kDa component ;mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae); Activator-recruited cofactor 36 kDa component; Vitamin D3 receptor-interacting protein complex 36 kDa component
Gene ID: 29079
mRNA Refseq: NM_014166
Protein Refseq: NP_054885
MIM: 605718
UniProt ID: Q9NPJ6
Chromosome Location: 13q14.2
Pathway: Gene Expression
Function: RNA polymerase II transcription mediator activity; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; receptor activity; thyroid hormone receptor binding; transcription activator activity; transcription cofactor activity; vitamin D receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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