||Recombinant Human folate hydrolase encoding 316-510 aa was expressed inE. coli.It was fused with a GST tag at the N-terminus.
||SOX9 is a member of the family of SOX (Sry-type high mobility group box) gene that was first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX9 by PKA increases its DNA binding and transcriptional activity. SOX9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibit the expression of genes for cartilage extracellular matrix proteins. The down-regulation of Sox9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
||100μl purified human Lyn protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.
||ELISA•Inhibition Assays• Western Blotting.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 48 kDa (including GST).
|Storage & Stability:
||Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.