||Human TNK1, catalytic domain [106-390 amino acids] was expressed as N-terminal GST-fusion protein (58 kDa) usingbaculovirusexpression system. GST-TNK1 was purified by using glutathione sepharose chromatography and gel filtration chromatography.
||TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC (MIM 190090) and JAK (see MIM 147795) families, mediate intracellular signaling downstream of receptor activation.
||83 %. The purity was assessed by SDS-PAGE/CBB staining.
||50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5.
||Store at -80℃. Avoid repeating freeze-thaws.