"Ctse" Related Products


Recombinant Mouse Cathepsin E, His-tagged

Cat. No. : Ctse-749M
Product Overview : Recombinant mouse cathepsin E was fused at the C-terminus to a His•Tag sequence and expressedNSO cells. MW = 44 kDa.
Description : Cathepsin E is an intracellular aspartic protease of the pepsin family that has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein (APP). Due to glycosylation the apparent molecular weight of the dimer is ~100 kDa under non-reducing conditions. Requires activation prior to use.
Source : NSO cells.
Purity : ≥95 by SDS-PAGE.
Form : Liquid. In 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered. Please refer to vial label for lot-specific concentration.
Recommended Reaction Conditions : Activation Protocol: Pre-incubate Cathepsin E for 15 min. at room temperature, at a concentration of 1 µg/ml in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl. Activity: Optimal activity is achieved in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl, room temperature.
Endotoxins : ≤1.0 EU/µg enzyme.
Specific Activity : ≥8 units/μg.
Unit Definition : One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH2 at 25°C, pH 3.5.
Toxicity : Standard Handling (MSDS available upon request).
Storage : ≤ -70℃. Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70℃).
Gene Name : Ctse cathepsin E [ Mus musculus ]
Synonyms : Ctse; cathepsin E; CE; CatE; A430072O03Rik; C920004C08Rik; cathepsin E; OTTMUSP00000025900; OTTMUSP00000025901
Gene ID : 13034
mRNA Refseq : NM_007799
Protein Refseq : NP_031825
UniProt ID : P70269
Chromosome Location : 1 F; 1 69.1 cM
Pathway : Lysosome
Function : aspartic-type endopeptidase activity; peptidase activity; protein homodimerization activity

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