||Recombinant rat insulin degrading enzyme was fused to a His•Tag sequence and expressed inS. frugiperdainsect cells.MW = 110 kDa.
||Insulin Degrading Enzyme (IDE) is a large zinc-binding protease of the M16A metalloprotease subfamily known to cleave multiple short polypeptides that vary considerably in sequence. IDE was first identified by its ability to degrade the B chain of the hormone insulin. This activity was observed over fifty years ago, though the enzyme specifically responsible for B chain cleavage was identified more recently. This discovery revealed considerable amino acid sequence homology between IDE and the previously characterized bacterial protease pitrilysin, suggesting a common proteolytic mechanism.
||≥90% by SDS-PAGE.
||Liquid. In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.
||≥3 U/mg protein.
||One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.
||≥4 units/mg protein.
||≤ -70℃. Avoid freeze/thaw.