Active Recombinant Human ALCAM protein, His-tagged

• Cat.No.: ALCAM-3140H
• Product Overview: Recombinant Human ALCAM (Met1-Ala526) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Immobilized human ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.1-0.22 μg/ml.
• Molecular Mass: The recombinant human ALCAM consists of 510 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, human ALCAM migrates as an approximately 78.3 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Ala526
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ALCAM activated leukocyte cell adhesion molecule [ Homo sapiens ]
• Official Symbol: ALCAM
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; activated leucocyte cell adhesion molecule; CD166 antigen; CD166; MEMD; FLJ38514; MGC71733
• Gene ID: 214
• mRNA Refseq: NM_001243280
• Protein Refseq: NP_001230209
• MIM: 601662
• UniProt ID: Q13740