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Active Recombinant Human ICAM3 protein, His-tagged

Cat.No. : ICAM3-3164H
Product Overview : Recombinant Human ICAM3 protein(CAA49369.1)(Met1-His485), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to ICAM3-coated plates (12.5 μg/mL , 100 μL/well), approximately >25% cells will adhere specifically.
Molecular Mass : The recombinant human ICAM3 consists of 467 amino acids after removal of the signal peptide and predicts a molecular mass of 50.8 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3 is approximately 100-120 kDa due to the glycosylation.
Protein Length : Met1-His485
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 92 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : ICAM3 intercellular adhesion molecule 3 [ Homo sapiens ]
Official Symbol : ICAM3
Synonyms : ICAM3; intercellular adhesion molecule 3; CD50; CDW50; ICAM R; ICAM-3; intercellular adhesion molecule-3; ICAM-R;
Gene ID : 3385
mRNA Refseq : NM_002162
Protein Refseq : NP_002153
MIM : 146631
UniProt ID : P32942

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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