||Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 16.7kDa (154aa).
||Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl.
||> 90% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.