Cat. No. : |
MAPK1-918H |
Product Overview : |
Recombinant Human MAPK1 protein (Met 1-Ser 360), fused with the GST tag at the N-terminus, was expressed inInsect cells. |
Description : |
Mitogen-activated protein kinase 1, also known as Extracellular signal-regulated kinase 2, ERK-2, and MAPK1, is a member of the protein kinase superfamily and MAP kinase subfamily. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. |
Source : |
Insect cells. |
Molecular Mass : |
The recombinant human ERK2/GST chimera consists of 585 amino acids and predicts a molecular mass of 67 kDa as estimated in SDS-PAGE under reducing conditions. |
Predicted N Terminal : |
Met. |
Purity : |
> 98 % as determined by SDS-PAGE. |
Formulation : |
Lyophilized from sterile 50mM Tris , 100 mM Nacl, 0.5mM PMSF, 10%Glycerol, pH 8.0. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Reconstitution : |
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
Storage And Stability : |
Samples are stable for up to twelve months from date of receipt at -700C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |