||Recombinant human MT1-MMP hemopexin domain is produced as a periplasmic protein in E. coli.The protein consists of MT1-MMP amino acid residues G292PNIC . . . CPSGG489 followed by the His-tag VTHHHHHH. The calculated Mr of the recombinant protein is 24 238 Da.
||Matrix metalloproteinases (MMPs) are Zn2+- and Ca2+-dependent endopeptidases which function in the turnover of extracellular matrix components. Presently fifteen secreted MMPs and six membranetype MMPs are known to be expressed in humans. MT1-MMP consists of 559 amino acid residues with a calculated Mr of 63 516 Da. The following domains and sequence regions are distinguished in MT1-MMP: Prodomain (Ser1 - Arg88), catalytic domain (Tyr89 - Gly261), junction between catalytic domain and hemopexin domain (Gly262 - Gly292), hemopexin-like domain (Pro293 - Cys485) and C-terminal sequence (Pro486 - Val559) with transmembrane segment. A soluble form of MT1-MMP without transmembrane segment has been found in culture medium of a breast carcinoma cell line.
||The protein is solubilized in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2.
||MT1-MMP hemopexin domain represents more than 95 % of total protein in the preparation.
||Isolated MT1-MMP hemopexin domain allows investigate interactions of this domain with low molecular weight ligands and with macromolecules. To detect and monitor recombinant hemopexin domain in such investigations the His-tag can be used. MT1-MMP hemopexin domain can also serve as antigen for generation of antibodies to MT1-MMP.
|Storage And Stability:
||MT1-MMP hemopexin domain is stable until the expiry date given on the label if stored at -70°C. The protein can be kept at -20°C for several weeks and in ice for several days. Repeated freezing and thawing should be avoided.