||Recombinant Full-length of human FER (1-822(end) amino acids) was expressed as N terminal GST-fusion protein (122 kDa) usingbaculovirusexpression system. GST-FER was purified by using glutathione sepharose chromatography and anion exchange chromatography.
||Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.
||94 %. The purity was assessed by SDS-PAGE/CBB staining.
||50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5.
||Store at -80℃. Avoid repeating freeze-thaws.