||Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate inE.coliand purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW = 20.7 kDa (179aa).
||Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factorsthat regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts.
|Amino Acid Sequence:
||MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN
||> 90% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0), 2mM DTT, 10% glycerol.
||< 1.0 EU per 1 μg of protein (determined by LAL method).
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.