"VRK1" Related Products

Expand
Expand
Expand

Recombinant Human Vaccinia Related Kinase 1, GST-His

Cat. No.: VRK1-706H
Product Overview: Recombinant Human VRK1, Amino acids M1-K396, N-terminally fused to GST-HIS6-Thrombin cleavage site, was produced inSf9cells.MW = 75,142 Da.
Description: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination.
Source: Baculovirus infected Sf9 cells.
Purification: One-step affinity purification using GSH-agarose.
Product Identity: VRK1, was confirmed as VRK1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
Concentration: 0.269 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
Specific Activity: 30 pmol/μg×min.
Storage: -80°C. Avoid repeated freeze-thaw cycles!
Gene Name: VRK1 vaccinia related kinase 1 [ Homo sapiens ]
Synonyms: VRK1; vaccinia related kinase 1; PCH1; MGC117401; MGC138280; MGC142070; serine/threonine-protein kinase VRK1; vaccinia-related kinase 1; vaccinia virus B1R-related kinase 1; EC 2.7.11.1
Gene ID: 7443
mRNA Refseq: NM_003384
Protein Refseq: NP_003375
MIM: 602168
UniProt ID: Q99986
Chromosome Location: 14q32
Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.