||Recombinant Human SRPK2, Amino acids M1-S688, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 107,422 Da.
||Serine/threonine-protein kinase SRPK2 is an enzyme that in humans is encoded by the SRPK2 gene. SRPK2 has been shown to interact with U2AF2 and ASF/SF2.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||SRPK2was confirmed as SRPK2 by specific Western Blotting using anti SRPK2 antibody.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.330 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!