||Recombinant Human NTRK1, C-terminal fragment, amino acids G443-G796, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells.MW = 69,726 Da.
||TrkA is the high affinity catalytic receptor for the neurotrophin, Nerve Growth Factor, or "NGF". As such, it mediates the multiple effects of NGF, which includes neuronal differentiation and survival.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||TRK-A was confirmed as TRK-A by by mass spectroscopy LC-ESIMS/MS (Protagen AG, Germany).
||50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.133 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!