||RecombinantHuman MELK, N-terminal fragment, amino acids M1-A342, untagged, was expressed inSf9insect cells. MW = 39,852 Da.
||Maternal embryonic leucine zipper kinase is an enzyme that in humans is encoded by the MELK gene.MELK has been shown to interact with CDC25B.
||Baculovirus infected Sf9 cells.
||GST-Affinity Chromatography followed by 3C mediated removal of the GST tag.
||MELK was confirmed as MELK by mass spectroscopy LC-ESI-MS/MS.
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol.
||0.191 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!