||Recombinant Human MST2, full length, amino acids M1-F491, untagged, expressed inSf9insect cells. MW = 56,884 Da.
||Serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the STK3 gene. Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions.
||Baculovirus infected Sf9 cells.
||GST-Affinity Chromatography followed by 3C mediated removal of the GST tag.
||MST2 was confirmed as MST2 by mass spectroscopy LC-ESI-MS/MS.
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol.
||0.271 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!