||Recombinant Human TBK1, full length, amino acids M1-L729, untagged, expressed inSf9insect cells. MW = 84,225 Da.
||The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors.
||Baculovirus infected Sf9 cells.
||TBK1 Lot 004 was confirmed as TBK1 by mass spectroscopy LC-ESI-MS/MS.
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol.
||0.086 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!