||RecombinantE. coliHolliday Junction DNA Helicase RuvC is expressed in E. coli. MW=19KDa.
||E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
||50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
||RuvC protein over 90% by SDS-PAGE (CBB staining).
||1) Studies on homologous recombination mechanism. 2) To use as endonuclease which functions specifically to the Holliday structure.
||1.0 mg/ml (determined by BCA method).