Recombinant E. coli Holliday Junction DNA Helicase RuvC

• Cat.No.: RuvC-2666E
• Product Overview: RecombinantE. coliHolliday Junction DNA Helicase RuvC is expressed in E. coli. MW=19KDa.

Specification

• Cat. No.: RuvC-2666E
• Description: E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
• Source: E. coli.
• Form: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
• Purity: RuvC protein over 90% by SDS-PAGE (CBB staining).
• Usage: 1) Studies on homologous recombination mechanism. 2) To use as endonuclease which functions specifically to the Holliday structure.
• Concentration: 1.0 mg/ml (determined by BCA method).
• Storage: -20⁰C.

Gene Information

• Gene Name: ruvC Crossover junction endodeoxyribonuclease ruvC [ Escherichia coli O55:H7 str. CB9615 ]
• Synonyms:
  ruvC; Crossover junction endodeoxyribonuclease ruvC
• Gene ID: 8871275
• Protein Refseq: YP_003499863
• Pathway: Homologous recombination