Recombinant Human Neuregulin 1

• Cat.No.: NRG1-2675H
• Product Overview: Recombinant Human NRG1 is expressed inE. coli.

Specification

• Cat. No.: NRG1-2675H
• Description: Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation.
• Source: E. coli.
• MW: 44kDa (full length) and 30kDa (extracellular domain).
• Purity: Over 95% pure by PAGE.
• Species Reactivity: Human and Mouse.
• Applications: • Western Blotting (As a positive control) • Stimulation of Tyrosine Kinase Activity of Erbs (Protein at 5nM) • Cell Proliferation and Differentiation (Protein at 5nM: dilute 5μg of stock protein to 30ml of culture medium).
• Positive Control: T47-D breast cancer cells.
• Formulation: Solution at 0.30 mg/ml in 20% glycerol.
• Activity: It may be used on the repair of RNA-polymerase II transcribed genes.
• Storage: Store vial at -20°C to -70°C. When stored at the recommended temperature, this protein is stable for 24 months.

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homo sapiens ]
• Synonyms:
  neuregulin 1; NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; pro-neuregulin-1; membrane-bound isoform; pro-NRG1; glial growth factor; neu differentiation factor; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); pro-NRG1
• Gene ID: 3084
• mRNA Refseq: NM_001159995
• Protein Refseq: NP_001153467
• MIM: 603013
• UniProt ID: Q02297
• Chromosome Location: 8p12
• Pathway: ErbB signaling pathway
• Function: cytokine activity; growth factor activity; protein binding; receptor tyrosine kinase binding; transcription cofactor activity; transmembrane receptor protein tyrosine kinase activator activity