Recombinant Rat Abcc2
Cat.No. : | Abcc2-2538R |
Product Overview : | Recombinant Rat Abcc2 was expressed inSf9 cells. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Abcc2-2538R |
Description : | MRP2 (ABCC2) is an organic anion transporter found in liver, kidney, and gut epithelium apical membranes. The transport of glucuronate conjugates plays a role in the detoxification of endogenous and xenobiotic substances, and may cause multidrug resistance (MDR) in tumor cells. The rat Mrp2 transporter shows 72.3% sequence identity and 85.6% sequence similarity with human MRP2. Both transporters are expressed on the canalicular membrane of the liver and are known to be responsible for the transport of some organic molecules and their conjugates to the bile. |
Source : | Sf9 cells. |
Form : | Supplied as isolated Sf9 cell membranes containing rat Mpr2 suspended in TMEP solution. |
Biochem/physiol Actions : | Detection of ATPase activity of the Mrp2 protein is a measure of transporter activity. The assay is performed using purified membrane vesicles from Sf9 (Spodoptera frugiperda) cells, expressing high levels of Mrp2 protein. ABC transporters pump substrates out of the cell by using hydrolysis of ATP as an energy source. ATP hydrolysis yields inorganic phosphate (Pi), which can be detected by a simple colorimetric reaction. The amount of Pi liberated is proportional to the activity of the transporter. |
Analysis Note : | Membrane preparations containing ABC transporters show a baseline ATPase activity that varies for different transporters. Transported substrates increase this baseline ATPase activity, while inhibitors or slowly transported compounds inhibit the baseline ATPase activity and/or the ATPase activity measured in the presence of a stimulating agent. Both activation and inhibition studies can be performed. |
Storage : | −70°C. |
Gene Name : | Abcc2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 [ Rattus norvegicus ] |
Synonyms : | Abcc2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; Mrp2; Cmoat; canalicular multispecific organic anion transporter 1; canalicular multidrug resistance protein; multidrug resistance associated protein 2; multidrug resistance-associated protein 2; ATP-binding cassette sub-family C member 2; calicular multispecific organic anion transporter |
Gene ID : | 25303 |
mRNA Refseq : | NM_012833 |
Protein Refseq : | NP_036965 |
UniProt ID : | Q63120 |
Chromosome Location : | 1q51 |
Pathway : | ABC transporters |
Function : | ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; nucleotide binding; organic anion transmembrane transporter activity TAS; protein domain specific binding; transporter activity |
Products Types
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (15)
Ask a questionCry1Ca is a protein that belongs to the family of crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis.Some reseachs show that Cry1Ca could decrease the transport function of ABCC2 in plant.
Carriage of the ABCC2 (MRP2) rs717620 T variant may increase the risk of hyperbilirubinemia and mortality in patients with DILI.
Yes, Dubin-Johnson syndrome (DJS) is a hereditary condition, which means it is passed down through families.
Causes Dubin-Johnson syndrome is caused by changes in a gene known as ABCC2. The ABCC2 gene provides instructions for making a protein that transports certain substances out of cells so they can be released (excreted) from the body.
ABCC2 and ABCC3 (also known as MRP2 and MRP3) mediate the transport of various conjugated organic anions, including many drugs, toxicants and endogenous compounds.
ABCC2/Abcc2 (MRP2/Mrp2) is expressed at major physiological barriers, such as the canalicular membrane of liver cells, kidney proximal tubule epithelial cells, enterocytes of the small and large intestine, and syncytiotrophoblast of the placenta.
ABCC2/Abcc2 transports a variety of amphiphilic anions that belong to different classes of molecules, such as endogenous compounds (e.g., bilirubin-glucuronides), drugs, toxic chemicals, nutraceuticals, and their conjugates.
The ABCC2 c.-24C>T polymorphism is a likely risk factor for resistance to AEDs.
Some researchs show that the ABCC2 G1249A polymorphism is significantly associated with a decreased risk of AED resistance. However, further functional investigations are warranted to validate the association.
Acquired or hereditary deficiency of ABCC2, the latter known as Dubin-Johnson syndrome in humans
It is possible that ABCC2/Abcc2 transport activity as well as inhibitor sensitivity could lead to a number of phenomena (e.g. a difference in the biliary excretion clearance, a delay in the elimination half-life from the circulating blood and toxic side effects on ABCC2 -mediated drug-drug interactions, such as drug-induced hyperbilirubinemia).
Human ABCC2 is an ATP-binding cassette transporter involved in the export of endobiotics and xenobiotics. It is involved in cisplatin resistance in cancer cells, particularly in ovarian cancer.
ABCC2 is a member of the multidrug resistance protein subfamily localized exclusively to the apical membrane domain of polarized cells, such as hepatocytes, renal proximal tubule epithelia, and intestinal epithelia.
The hepatic ABCC2 pump contributes to the driving forces of bile flow.
Customer Reviews (3)
Write a reviewComplete packaging and fast transportation
Good positioning effect
Less clutter and clear background
Ask a Question for All Abcc2 Products
Required fields are marked with *
My Review for All Abcc2 Products
Required fields are marked with *
Inquiry Basket