Dihydrofolate Reductase Colorimetric Activity Kit

• Cat.No.: Kit-2097
• Product Overview: Dihydrofolate Reductase assay kit is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96- well plate. The assay is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples.

Specification

• Size: 100 assays
• Applications: Measurement of Dihydrofolate Reductase activity in various tissues/cellsAnalysis of folate metabolism
• Storage: Upon receiving the kit, store DHFR substrate at -80°C. Store other components at -20°C. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay. Upon opening, use within two months.DHFR Assay Buffer: Warm to room temperature before use. Store at 4ºC or -20°C.DHFR Substrate: Aliquot and store at -80°C, protected from light. Avoid repeated freeze/thaw cycles.Dihydrofolate Reductase: Store at -20°C. Avoid repeated freeze/thaw cycles. Keep on ice while in use.NADPH: Reconstitute with 200 μl DHFR Assay Buffer to generate 20 mM NADPH Stock Solution. Aliquot and store at –20°C. Keep on ice while in use.