|Product Overview:||GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 22.214.171.124) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 μU/mL in a variety of samples.|
|Applications:||Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells;
Analysis ofglycolysis and pentose phosphate pathways.
|Kit Components:||GAPDH Assay Buffer; GAPDH Substrate; GAPDH Developer; NADH Standard (500 nmol); GAPDH Positive Control|
|Detection method:||Absorbance (450 nm)|
|Features & Benefits:||Simple, rapid & convenient; can measure GAPDH activityas low as 100 μU/mLin a variety of samples|