GAPDH Activity Assay Kit
Cat.No. : | Kit-2216 |
Product Overview : | GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 μU/mL in a variety of samples. |
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Applications : | Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells; Analysis ofglycolysis and pentose phosphate pathways. |
Storage : | -20°C |
Size : | 100 assays |
Products Types
◆ Recombinant Protein | ||
GAPDH-014H | Recombinant Human GAPDH Protein(Met 1-Glu 335), His-tagged | +Inquiry |
Gapdh-3150M | Recombinant Mouse Gapdh Protein, Myc/DDK-tagged | +Inquiry |
Gapdh-1562R | Recombinant Rat Gapdh Protein, His-tagged | +Inquiry |
GAPDH-045H | Recombinant Human GAPDH Protein, His-tagged | +Inquiry |
GAPDH-2191H | Recombinant Human GAPDH Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
GAPDH-62H | Native Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) | +Inquiry |
GAPDH-126R | Active Native Rabbit Glyceraldehyde-3-phosphate Dehydrogenase | +Inquiry |
◆ Lysates | ||
GAPDH-6024HCL | Recombinant Human GAPDH 293 Cell Lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewCustomer service was patient, prompt and accurate in answering my queries.
High protein concentration, good quality, worth buying!
The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.
Q&As (5)
Ask a questionGAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.
GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.
GAPDH binds to HDAC1.
GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.
Interference with GAPDH protein expression results in telomere shortening.
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