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GAPDH Activity Assay Kit

Cat.No. : Kit-2216
Product Overview : GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 μU/mL in a variety of samples.
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Applications : Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells;
Analysis ofglycolysis and pentose phosphate pathways.
Storage : -20°C
Size : 100 assays

Not For Human Consumption!

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07/14/2022

    Customer service was patient, prompt and accurate in answering my queries.

    11/26/2021

      High protein concentration, good quality, worth buying!

      01/02/2021

        The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

        Q&As (5)

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        Is GAPDH expression stable across tissues? 08/12/2022

        GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

        What diseases has GAPDH been closely associated with the study of? 07/14/2022

        GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

        What is the relationship between GAPDH and HDAC1? 01/25/2022

        GAPDH binds to HDAC1.

        What are the biological functions of GAPDH? 01/01/2022

        GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

        What are the effects of affecting protein expression of GAPDH? 03/05/2021

        Interference with GAPDH protein expression results in telomere shortening.

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