Recombinant Chicken GAPDH Protein (2-333 aa), His-tagged

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Recombinant Chicken GAPDH Protein (2-333 aa), His-tagged

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Cat.No. : GAPDH-517C
Product Overview : Recombinant Chicken GAPDH Protein (2-333 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description : Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assbly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins.
Source : E. coli
Species : Chicken
Tag : His
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 39.6 kDa
Protein length : 2-333 aa
AA Sequence : VKVGVNGFGR IGRLVTRAAV LSGKVQVVAI NDPFIDLNYM VYMFKYDSTH GHFKGTVKAE NGKLVINGHA ITIFQERDPS NIKWADAGAE YVVESTGVFT TMEKAGAHLK GGAKRVIISA PSADAPMFVM GVNHEKYDKS LKIVSNASCT TNCLAPLAKV IHDNFGIVEG LMTTVHAITA TQKTVDGPSG KLWRDGRGAA QNIIPASTGA AKAVGKVIPE LNGKLTGMAF RVPTPNVSVV DLTCRLEKPA KYDDIKRVVK AAADGPLKGI LGYTEDQVVS CDFNGDSHSS TFDAGAGIAL NDHFVKLVSW YDNEFGYSNR VVDLMVHMAS KE
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Gallus gallus (chicken) ]
Official Symbol : GAPDH
Synonyms : KNC-NDS6;
Gene ID : 374193
mRNA Refseq : NM_204305
Protein Refseq : NP_989636
UniProt ID : P00356

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