GMP Recombinant Human BDNF Protein
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| Cat.No. : | BDNF-113HG |
| Product Overview : | Recombinant Human BDNF, His129-Arg247, was produced in Sf 21 (baculovirus)-derived in an animal component free process under cGMP guidelines. |
| Source : | Insect cell |
| Species : | Human |
| Form : | Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl. |
| Bio-activity : | Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10^3 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534). |
| Molecular Mass : | Predicted Molecular Mass: 13.5 kDa SDS-PAGE: 13-14 kDa, reducing conditions |
| N-terminal Sequence Analysis : | His129-Ser -Asp-Pro-A la-Arg-Arg -Gly-Glu-L eu |
| Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : | >97%, by SDS-PAGE with silver staining, under reducing conditions. |
| Storage : | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : | Reconstitute at 100 μg/mL in PBS. |
| Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
| Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
| Gene Name : | BDNF brain-derived neurotrophic factor [ Homo sapiens ] |
| Official Symbol : | BDNF |
| Synonyms : | BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632; |
| Gene ID : | 627 |
| mRNA Refseq : | NM_001143805 |
| Protein Refseq : | NP_001137277 |
| MIM : | 113505 |
| UniProt ID : | P23560 |
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◆ Lysates
| BDNF-001MCL | Recombinant Mouse BDNF cell lysate | +Inquiry |
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