|Product Overview:||Recombinant Human BDNF, His129-Arg247, was produced in Sf 21 (baculovirus)-derived in an animal component free process under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.|
|Bio-activity:||Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10^3 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
|Molecular Mass:||Predicted Molecular Mass: 13.5 kDa
SDS-PAGE: 13-14 kDa, reducing conditions
|N-terminal Sequence Analysis:||His129-Ser-Asp-Pro-Ala-Arg-Arg-Gly-Glu-Leu|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||BDNF brain-derived neurotrophic factor [ Homo sapiens ]|
|Synonyms:||BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632;|