GMP Recombinant Human BDNF Protein BDNF-113HG

Specification
Gene Information
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Cat.No.:	BDNF-113HG
Product Overview:	Recombinant Human BDNF, His129-Arg247, was produced in Sf 21 (baculovirus)-derived in an animal component free process under cGMP guidelines.
Source:	Insect cell
Species:	Human
Form:	Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Bio-activity:	Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10^3 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
Molecular Mass:	Predicted Molecular Mass: 13.5 kDa SDS-PAGE: 13-14 kDa, reducing conditions
N-terminal Sequence Analysis:	His129-Ser-Asp-Pro-Ala-Arg-Arg-Gly-Glu-Leu
Endotoxin:	<0.10 EU per 1 μg of the protein by the LAL method.
Purity:	>97%, by SDS-PAGE with silver staining, under reducing conditions.
Storage:	Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:	Reconstitute at 100 μg/mL in PBS.
Quality Statement:	Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping:	The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

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Gene Name:	BDNF brain-derived neurotrophic factor [ Homo sapiens ]
Official Symbol:	BDNF
Synonyms:	BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632;
Gene ID:	627
mRNA Refseq:	NM_001143805
Protein Refseq:	NP_001137277
MIM:	113505
UniProt ID:	P23560