GMP Recombinant Human BMP2 Protein
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Cat.No. : | BMP2-119HG |
Product Overview : | Recombinant Human BMP2, Gln283-Arg396, was produced in CHO cell under cGMP guidelines. |
Source : | CHO cell |
Species : | Human |
Form : | Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid. |
Bio-activity : | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL. The specific activity of recombinant human BMP-2 is approximately 781 units/μg, which is calibrated against human BMP-2 WHO Standard (NIBSC code: 93/574). |
Molecular Mass : | Predicted Molecular Mass: 13 (monomer) kDa SDS-PAGE: 15-16 kDa, reducing conditions |
N-terminal Sequence Analysis : | Gln-Ala-Ly s-His-Lys- Gln-Arg-Ly s-Arg-Leu |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE with silver staining, under reducing conditions. |
Storage : | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 100 μg/mL in 4 mM HCl. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Gene Name : | BMP2 bone morphogenetic protein 2 [ Homo sapiens ] |
Official Symbol : | BMP2 |
Synonyms : | BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2; |
Gene ID : | 650 |
mRNA Refseq : | NM_001200 |
Protein Refseq : | NP_001191 |
MIM : | 112261 |
UniProt ID : | P12643 |
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◆ Lysates
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