|Product Overview:||Recombinant Human BMP2, Gln283-Arg396, was produced in CHO cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.|
|Bio-activity:||Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
The specific activity of recombinant human BMP-2 is approximately 781 units/μg, which is calibrated against human BMP-2 WHO Standard (NIBSC code: 93/574).
|Molecular Mass:||Predicted Molecular Mass: 13 (monomer) kDa
SDS-PAGE: 15-16 kDa, reducing conditions
|N-terminal Sequence Analysis:||Gln-Ala-Lys-His-Lys-Gln-Arg-Lys-Arg-Leu|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in 4 mM HCl.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||BMP2 bone morphogenetic protein 2 [ Homo sapiens ]|
|Synonyms:||BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2;|