|Product Overview:||Recombinant Human EGF, Asn971-Arg1023, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20‑100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 10^3 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
|Molecular Mass:||Predicted Molecular Mass: 6 kDa
SDS-PAGE: 6 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 200 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||EGF epidermal growth factor [ Homo sapiens ]|
|Synonyms:||EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4;|