|Product Overview:||Recombinant Human FGF7, Cys32-Thr194, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.
The specific activity of recombinant human KGF/FGF-7 is approximately 1.3 x 10^3 U/μg, which is calibrated against recombinant human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).
|Molecular Mass:||Predicted Molecular Mass: 19 kDa
SDS-PAGE: 20 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-(Cys)32-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||FGF7 fibroblast growth factor 7 [ Homo sapiens ]|
|Synonyms:||FGF7; fibroblast growth factor 7; keratinocyte growth factor; KGF; FGF-7; heparin-binding growth factor 7; HBGF-7;|