|Product Overview:||Recombinant Human HPN, Arg45-Leu417 (Asp161Glu & Arg162Lys), with a C-terminal 10-His tag was produced in NS0 cell under cGMP guidelines.|
|Form:||Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.|
|Bio-activity:||Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >20,000 pmol/min/μg.|
|Molecular Mass:||Predicted Molecular Mass: 42 kDa
SDS-PAGE: 40-43 kDa, reducing conditions
|N-terminal Sequence Analysis:||Arg45-Ser-Asp-Gln-Glu-Pro-Leu-Tyr-Pro-Val|
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Gene Name:||HPN hepsin [ Homo sapiens ]|
|Synonyms:||HPN; hepsin; serine protease hepsin; TMPRSS1; transmembrane protease; serine 1; transmembrane protease serine 1; serine protease hepsin catalytic chain; serine protease hepsin non-catalytic chain;|
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