Active GMP Recombinant Human HPN Protein

• Cat.No.: HPN-147HG
• Product Overview: Recombinant Human HPN, Arg45-Leu417 (Asp161Glu & Arg162Lys), with a C-terminal 10-His tag was produced in NS0 cell under cGMP guidelines.

Specification

• Source: Mammalian cells
• Species: Human
• Form: Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
• Bio-activity: Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >20,000 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 42 kDa
  SDS-PAGE: 40-43 kDa, reducing conditions
• N-terminal Sequence Analysis: Arg45-Ser-Asp-Gln-Glu-Pro-Leu-Tyr-Pro-Val
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.

Gene Information

• Gene Name: HPN hepsin [ Homo sapiens ]
• Official Symbol: HPN
• Synonyms: HPN; hepsin; serine protease hepsin; TMPRSS1; transmembrane protease; serine 1; transmembrane protease serine 1; serine protease hepsin catalytic chain; serine protease hepsin non-catalytic chain
• Gene ID: 3249
• mRNA Refseq: NM_002151
• Protein Refseq: NP_002142
• MIM: 142440
• UniProt ID: P05981