|Product Overview:||Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC-8 activity Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.|
|Applications:||Suitable for high throughput measurement of HDAC8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.|
|Kit Components:||HDAC-8 Assay Buffer; HDAC-8 Substrate; HDAC-8 Positive Control; AFC Standard (1 mM); Developer; Trichostatin A (10 μM)|
|Detection method:||Fluorescence (Ex/Em = 380/500 nm)|
|Features & Benefits:||Simple procedure; takes ~ 60 min;
Fast and convenient;
Kit contains the necessary reagents for accurate measurement of HDAC-8 activity
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