Human Apolipoprotein C-II

• Cat.No.: APOC2-4773H
• Product Overview: Product is 0.5 mg human APOC2 lyophilized from 0.01M ammonium bicarbonate. Very low density lipoproteins (VLDL) are isolated from fresh human plasma by ultracentrifugation. The VLDL are delipidated and the APOC2 is purified by using proprietary column chromatographic methds.

Specification

• Cat. No.: APOC2-4773H
• Description: The protein encoded by this gene is secreted in plasma where it is a component of very low density lipoproteins and chylomicrons. This protein activates the enzyme lipoprotein lipase in capillaries[1], which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis.
• Source: Purified
• Species: Human
• Properties: APOC2, which has a Mr of 8.8 kD, is a cofactor for lipoprotein lipase, the enzyme that catalyzes the hydrolysis of triglycerides. Due to its amphipathic structure, APOC2 self-associates into oligomers at high protein concentrations. At concentrations above 1 mg/ml there may be little or no free monomer in the solution. APOC2 exists as monomers in buffers containing 5 M guanidine-HCl or 6 M urea.
• Specifications: Purity and identity are at least 98% as determined by quantitative laser densitometry of Coomassie Blue-stained SDS- and alkaline urea polyacrylamide gels. Protein is determined by the Pierce BCA Method, which was found to be comparable to the Lowry method, using 1% SDS with BSA as the standard.
• Reconstitution: To ensure complete solubilization and to minimize self-association and aggregation, the lyophilized powder should be dissolved in 5M guanidine-HCl. The buffer then may be changed by dilution, dialysis or gel filtration. Freezing solutions of apolipoproteins in the absence of denaturants favors self-association or aggregation.
• Storage: Store unopened product at -20°C or below. Store reconstituted material at 2-8°C no longer than 4 weeks.
• Pathways: Chylomicron-mediated lipid transport; HDL-mediated lipid transport; Lipid digestion; Lipoprotein metabolism; Metabolism of lipids and lipoproteins; Statin Pathway

Gene Information

• Gene Name: APOC2 apolipoprotein C-II [ Homo sapiens ]
• Official Symbol: APOC2
• Synonyms: APOC2; apolipoprotein C-II; APO-CII; APOC-II; MGC75082; apolipoprotein C-II; apolipoprotein C2; Apolipoprotein C-II; Apolipoprotein C2
• Gene ID: 344
• mRNA Refseq: NM_000483
• Protein Refseq: NP_000474
• MIM: 608083
• UniProt ID: P02655
• Chromosome Location: 19q13.2
• Function: enzyme activator activity; lipase inhibitor activity; lipid binding; lipoprotein lipase activator activity; phospholipase activator activity; phospholipase binding; protein homodimerization activity