Human C3 Knockdown Cell Lysate

• Cat.No.: C3-078HKCL
• Product Overview: WB-validated C3 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 187 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg C3 KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: C3 complement component 3 [ Homo sapiens (human) ]
• Official Symbol: C3
• Synonyms: C3; complement component 3; complement C3; CPAMD1; complement component C3; acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; ASP; AHUS5; ARMD9
• Gene ID: 718
• mRNA Refseq: NM_000064
• Protein Refseq: NP_000055
• MIM: 120700
• UniProt ID: P01024