Human CHD3 Knockdown Cell Lysate

• Cat.No.: CHD3-108HKCL
• Product Overview: WB-validated CHD3 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 226 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg CHD3 KD HeLa cell lysate
• Protein Families: Druggable Genome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: CHD3 chromodomain helicase DNA binding protein 3 [ Homo sapiens (human) ]
• Official Symbol: CHD3
• Synonyms: CHD3; chromodomain helicase DNA binding protein 3; chromodomain-helicase-DNA-binding protein 3; Mi 2a; Mi2 ALPHA; ZFH; hZFH; CHD-3; zinc finger helicase; ATP-dependent helicase CHD3; mi-2 autoantigen 240 kDa protein; zinc-finger helicase (Snf2-like); Mi-2a; Mi2-ALPHA
• Gene ID: 1107
• mRNA Refseq: NM_001005271
• Protein Refseq: NP_001005271
• MIM: 602120
• UniProt ID: Q12873