Recombinant Human CHD3 Protein, His-tagged

• Cat.No.: CHD3-450H
• Product Overview: Recombinant Human CHD3(Cys1551~Phe1742) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 25.7kDa
• Identity: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Protein length: Cys1551~Phe1742
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: CHD3 chromodomain helicase DNA binding protein 3 [ Homo sapiens ]
• Official Symbol: CHD3
• Synonyms: CHD3; chromodomain helicase DNA binding protein 3; chromodomain-helicase-DNA-binding protein 3; Mi 2a; Mi2 ALPHA; ZFH; hZFH; CHD-3; zinc finger helicase; ATP-dependent helicase CHD3; mi-2 autoantigen 240 kDa protein; zinc-finger helicase (Snf2-like); Mi-2a; Mi2-ALPHA
• Gene ID: 1107
• mRNA Refseq: NM_001005271
• Protein Refseq: NP_001005271
• MIM: 602120
• UniProt ID: Q12873