Human GRB2 Knockdown Cell Lysate

• Cat.No.: GRB2-184HKCL
• Product Overview: WB-validated GRB2 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 25 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg GRB2 KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Renal cell carcinoma, T cell receptor signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: GRB2 growth factor receptor-bound protein 2 [ Homo sapiens (human) ]
• Official Symbol: GRB2
• Synonyms: GRB2; growth factor receptor-bound protein 2; NCKAP2; HT027; protein Ash; SH2/SH3 adapter GRB2; abundant SRC homology; growth factor receptor-bound protein 3; epidermal growth factor receptor-binding protein GRB2; ASH; Grb3-3; MST084; MSTP084; EGFRBP-GRB2
• Gene ID: 2885
• mRNA Refseq: NM_002086
• Protein Refseq: NP_002077
• MIM: 108355
• UniProt ID: P62993