Human USP5 Knockdown Cell Lysate

• Cat.No.: USP5-466HKCL
• Product Overview: WB-validated USP5 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 96 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg USP5 KD HeLa cell lysate
• Protein Families: Druggable Genome, Protease
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: USP5 ubiquitin specific peptidase 5 (isopeptidase T) [ Homo sapiens (human) ]
• Official Symbol: USP5
• Synonyms: USP5; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); ubiquitin carboxyl-terminal hydrolase 5; IsoT; isopeptidase T; ubiquitin isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ISOT
• Gene ID: 8078
• mRNA Refseq: NM_001098536
• Protein Refseq: NP_001092006
• MIM: 601447
• UniProt ID: P45974