Human VPS35 Knockdown Cell Lysate

• Cat.No.: VPS35-468HKCL
• Product Overview: WB-validated VPS35 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 92 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg VPS35 KD HeLa cell lysate
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: VPS35 vacuolar protein sorting 35 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: VPS35
• Synonyms: VPS35; vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast); vacuolar protein sorting-associated protein 35; FLJ10752; MEM3; hVPS35; maternal-embryonic 3; PARK17; FLJ13588; FLJ20388; DKFZp434E1211; DKFZp434P1672
• Gene ID: 55737
• mRNA Refseq: NM_018206
• Protein Refseq: NP_060676
• MIM: 601501
• UniProt ID: Q96QK1