Mouse Anti-Human Carbonic Anhydrase XII Monoclonal Antibody

Cat.No. : CAB11484MH
Product Overview : Mouse monoclonal antibody to human carbonic anhydrase XII.
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Antigen Description : Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. This bitopic membrane protein contains an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain. The structure reveals a prototypical CA fold and two CA XII domains associate to form an isologous dimer. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker.
Specificity : Human Carbonic Anhydrase XII / CA12. No cross-reactivity in ELISA with Mouse Carbonic Anhydrase XII / CA12, Human Carbonic Anhydrase II / CA2, Human Carbonic Anhydrase III / CA3, Human Carbonic Anhydrase IV / CA4, Human Carbonic Anhydrase VA / CA5A, Human Carbonic Anhydrase VB / CA5B, Human Carbonic Anhydrase VIII / CA8, Human Carbonic Anhydrase X / CA10, Human Carbonic Anhydrase XIII / CA13, Human Carbonic Anhydrase XIV / CA14, Human Carbonic Anhydrase IX / CA9, Human cell lysate (293 cell line).
Immunogen : Recombinant Human Carbonic Anhydrase XII / CA12 Protein
Host : Mouse
Isotype : Mouse IgG1
Species : Human
Clone : 3E7F2
Purification : Protein A affinity chromatography
Applications : Western Blot; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect CA12 in WB. Using a DAB detection system, the detection limit for CA12 is approximately 8 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect CA12-His. The detection limit for CA12-His is approximately 0.0195 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Carbonic Anhydrase XII / CA12. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CA12 carbonic anhydrase XII [ Homo sapiens ]
Official Symbol : CA12
Synonyms : CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151;
Gene ID : 771
mRNA Refseq : NM_001218
Protein Refseq : NP_001209
MIM : 603263
UniProt ID : O43570
Chromosome Location : 15q22
Function : carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known inhibitors specific to CA12, and how do they impact its enzymatic function? 07/13/2021

Known inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.

In which specific cellular compartments is CA12 predominantly localized, and how does this localization influence its role? 06/12/2020

Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.

What role does CA12 play in tumorigenesis, especially in hypoxic tumor environments? 08/29/2019

CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.

What downstream signaling pathways are activated or inhibited by CA12? 07/28/2019

Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.

How does CA12 activity get modulated under varying pH conditions? 04/08/2018

The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.

How does CA12 expression vary in response to different cellular stressors or stimuli? 03/08/2018

Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.

How do genetic variants of the CA12 gene influence its protein structure and function? 10/18/2017

Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.

Customer Reviews (3)

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12/20/2022

    Professional assistance, invaluable for complex protein experiments.

    05/09/2021

      Cost-effective quantitation, ideal for budget-conscious labs.

      11/21/2020

        Impressive expertise, accelerates our research progress.

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