Active Recombinant Human CA12 Protein, His-tagged

Cat.No. : CA12-971H
Product Overview : Recombinant Human CA12 (Accession # O43570) Ala25-Gln291, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Ala25
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its esterase activity. The specific activity is >10 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human CA12, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 38-41 kDa, reducing conditions.
Protein length : Ala25-Gln291
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : CA12 carbonic anhydrase XII [ Homo sapiens ]
Official Symbol : CA12
Synonyms : CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151;
Gene ID : 771
mRNA Refseq : NM_001218
Protein Refseq : NP_001209
MIM : 603263
UniProt ID : O43570
Chromosome Location : 15q22

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known inhibitors specific to CA12, and how do they impact its enzymatic function? 07/13/2021

Known inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.

In which specific cellular compartments is CA12 predominantly localized, and how does this localization influence its role? 06/12/2020

Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.

What role does CA12 play in tumorigenesis, especially in hypoxic tumor environments? 08/29/2019

CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.

What downstream signaling pathways are activated or inhibited by CA12? 07/28/2019

Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.

How does CA12 activity get modulated under varying pH conditions? 04/08/2018

The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.

How does CA12 expression vary in response to different cellular stressors or stimuli? 03/08/2018

Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.

How do genetic variants of the CA12 gene influence its protein structure and function? 10/18/2017

Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.

Customer Reviews (3)

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Reviews
12/20/2022

    Professional assistance, invaluable for complex protein experiments.

    05/09/2021

      Cost-effective quantitation, ideal for budget-conscious labs.

      11/21/2020

        Impressive expertise, accelerates our research progress.

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