Native Bovine CAPN2

• Cat.No.: CAPN2-350B
• Product Overview: CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.

Specification

• Source: Bovine Myocardium.
• Species: Bovine
• Form: 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.
• Molecular Mass: CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: his protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1,000). B- Add 1μg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
• Stability: CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: CAPN2 calpain 2, (m/II) large subunit [ Bos taurus (cattle) ]
• Official Symbol: CAPN2
• Synonyms: calpain-2 catalytic subunit; CANP 2; M-calpaincalpain M-type; millimolar-calpain; calpain-2 large subunit; calpain, large polypeptide L2; calcium-activated neutral proteinase 2
• Gene ID: 281662
• mRNA Refseq: NM_001103086
• Protein Refseq: NP_001096556
• UniProt ID: Q27971
• Chromosome Location: chromosome: 16
• Pathway: Alzheimer"s disease, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; calcium-dependent cysteine-type endopeptidase activity