Recombinant Human CAPN2 293 Cell Lysate

• Cat.No.: CAPN2-7863HCL

Specification

• Description: Antigen standard for calpain 2, (m/II) large subunit (CAPN2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CAPN2 calpain 2, (m/II) large subunit [ Homo sapiens ]
• Official Symbol: CAPN2
• Synonyms: CAPN2; calpain 2, (m/II) large subunit; calpain-2 catalytic subunit; CANPL2; CANPml; mCANP; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2; CANP2; FLJ39928
• Gene ID: 824
• mRNA Refseq: NM_001146068
• Protein Refseq: NP_001139540
• MIM: 114230
• UniProt ID: P17655
• Chromosome Location: 1q41-q42
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; ErbB1 downstream signaling, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem
• Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; cysteine-type peptidase activity; cytoskeletal protein binding; peptidase activity; protein binding; protein heterodimerization activity