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Native Bovine GSN Protein

Cat.No.: GSN-875B
Product Overview: Native Bovine GSN was purified from Bovine Plasma.
Description: Plasma gelsolin has a molecular mass of 83kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end. Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two binding sites for PIP2.
Source: Bovine Plasma
Species: Bovine
Form: 10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and 1% disaccharides, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.
Bio-activity: Fragmentation activity: The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25 centigrade. The specific viscosity of F-actin (1mg/ml) is reduced by ~65% after addition of gelsolin at a molar ratio of 1:100. The gelsolin stock solution was used as described below containing 1% disaccharides. F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin.
Molecular Mass: 83kDa
Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Storage: Gelsolin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 5 days. Storage in glycerol at -20 centigrade results in a significant decrease of the activity, and has to be determined individually.
Shipping: At ambient temperature. Upon delivery store at -70 centigrade.
Gene Name: GSN gelsolin [ Bos taurus ]
Official Symbol: GSN
Synonyms: ADF; actin-depolymerizing factor; brevin; gelsolin (amyloidosis, Finnish type)
Gene ID: 535077
mRNA Refseq: NM_001034627
Protein Refseq: NP_001029799
UniProt ID: Q3SX14

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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