|Product Overview:||This product is the Agarose bound Griffonia Simplicifolia Lectin II and has sugar specificity against N-Acetylglucosamine.|
|Description:||This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide|
|Bio-activity:||Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine|
|Molecular Mass:||113 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
Recommended conditions to elute bound glycoconjugates: 0.2M N-acetylglucosamine in buffered saline or Chitin Hydrolysate
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||3.0 mg/ml of settled gel|
|Synonyms:||Lectin; GSL II; BSL II|
Price does not include shipping and packaging costs.Request quote for final price.