Active Native Griffonia Simplicifolia Lectin II Protein, Agarose bound

• Cat.No.: Lectin-1787G
• Product Overview: This product is the Agarose bound Griffonia Simplicifolia Lectin II and has sugar specificity against N-Acetylglucosamine.

Specification

• Species: Griffonia Simplicifolia
• Source: Griffonia Simplicifolia
• Tag: Non
• Description: This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.
• Form: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide
• Bio-activity: Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine
• Molecular Mass: 113 kDa
• Applications: Glycobiology, Affinity Chromatography
• Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
  Recommended conditions to elute bound glycoconjugates: 0.2M N-acetylglucosamine in buffered saline or Chitin Hydrolysate
• Storage: Refrigerate. DO NOT FREEZE.
• Concentration: 3.0 mg/ml of settled gel

Gene Information

• Synonyms: Lectin; GSL II; BSL II
• UniProt ID: Q41263