| Species : |
Staphylococcus aureus |
| Description : |
α -hemolysin is a pore-forming cytotoxin, which is an extracellular protein secreted by most pathogenic Staphylococcus aureus strains. It is secreted in the form of water-soluble monomer and is a small β barrel protein. |
| Form : |
Lyophilized powder |
| Bio-activity : |
≥10,000 units/mg protein |
| Purity : |
~60 % by Lowry |
| Applications : |
1. As a component of electrolyte solution, it is used to test the pore formation in lipid bilayer by electrophysiological measurement;
2. The osteogenic inhibition of BMSCs was tested;
3. Preparation of α hemolysin molecularly imprinted polymer (MIP) for Biacore and surface plasmon resonance.
4. α -hemolysin was used in a study to test the efflux pump and hemolysin activity of Escherichia coli in dairy products. It is also used to test its adaptability to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation. |
| Storage : |
2-8 centigrade |
| Concentration : |
≥10,000 units/mg protein |
| CAS No. : |
94716-94-6 |
| Hemolysis unit : |
A hemolysis unit was frozen at 37 centigrade for 30 minutes, and then at 4 centigrade for 30 minutes. After that, 1% rabbit red blood cells suspended in phosphate buffered saline with pH 7.0 and containing 1% bovine serum albumin were 50% dissolved. |
