CAB11561RH
Optional Service: Optional requirements on this protein
Product Overview :
Rabbit Monoclonal Antibody to Human Interleukin 17 Receptor A
Antigen Description :
IL17RA ( Interleukin 17 receptor A ), also known as IL17R and CDw217, is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A (IL17A). IL17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. IL17A binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. IL17RA is a widely expressed glycoprotein that plays a central role in inflammatory responses. IL17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL17RA is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL17RA is 69% identical to the mouse IL17R. Within the extracellular domain, human IL17RA shares 72% aa sequence identity with mouse and rat IL17RA. IL17RA associates with IL17RC to form a signaling receptor complex for IL17 and IL17F. Ligand and IL17RA ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple proinflammatory chemokines.
Specificity :
Human IL17RA / CD217. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen :
Recombinant human IL17RA protein
Host :
Rabbit
Isotype :
Rabbit IgG
Species :
Human
Cross Reactivity :
012
Clone :
A013
Applications :
WB; ELISA
Dilution :
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human IL17RAin WB. Using a DAB detection system, the detection limit for human IL17RA is approximately 0.125 ng/lane under non-reducing conditions and reducing conditionsDirect ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human IL17RA. The detection limit for human IL17RA is approximately 0.00245 ng/well
Preparation :
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human IL17RA ( rh IL17RA ; NP_055154.3 ; Met 1 - Trp 320 ).
Format :
0.2 μm filtered solution in PBS with 5% trehalose
Storage :
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.