Recombinant Active Human IL24 Protein, His-tagged(C-ter)

• Cat.No.: IL24-180H
• Product Overview: Recombinant Active Human IL24 Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Source: E. coli
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human IL-20R alpha and IL-20R beta. The ED50 for this effect is < 100 pg/ml.
• AA Sequence: MQEFHFGPCQVKGVVPQKLWEAFWA VKDTMQAQDNITSARLLQQEVLQNV SDAESCYLVHTLLEFYLKTVFKNYH NRTVEVRTLKSFSTLANNFVLIVSQ LQPSQENAESCYLVHTLLEFYLKTV FKNYHNRTVEVRTLKSFSTLANNFV LIVSQ LQPSQEN
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 95% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 8.0)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL24 interleukin 24 [ Homo sapiens ]
• Official Symbol: IL24
• Synonyms: IL24; interleukin 24; ST16; interleukin-24; C49A; FISP; IL 4 induced secreted protein; IL 24; IL10B; mda 7; melanoma differentiation association protein 7; Mob 5; suppression of tumorigenicity 16 (melanoma differentiation); melanocyte-associated Mda-7; IL-4-induced secreted protein; melanoma differentiation-associated gene 7 protein; MDA7; MOB5
• Gene ID: 11009
• mRNA Refseq: NM_001185156
• Protein Refseq: NP_001172085
• MIM: 604136
• UniProt ID: Q13007

Reviews

07/07/2022

Very high quality, very reliable.

02/03/2021

Very pure, safe to use.

11/17/2019

High stability, can be stored for a long time.

Q&As

Are there side effects of Il24?

The current study shows that the side effects of Il24 are relatively small, and mainly reflected in local reactions, allergic reactions, etc.

Is Il24 safer and more effective than other treatments?

The safety and efficacy of Il24 need to be validated through more clinical studies, and the safety and efficacy of specific applications need to be evaluated based on individual patient differences.

Which molecules does Il24 interact with?

Il24 interacts with IL20 receptor complex, IL22 protein, etc.

How does Il24 interfere with the immune system?

It mainly regulates or interferes with the relevant processes of the immune system by inhibiting some mediators secreted by immune cells, such as cytokines and chemokines.

What are the advantages of Il24 application?

Il24 has good biological activity, stability and repeatability, and can be large-scale produced by genetic engineering technology, which has potential drug development and application prospects.

Can recombinant Il24 be used to treat tumors?

Yes, Il24 has been shown to treat many types of solid tumors, including brain glioma, melanoma, lung cancer, prostate cancer, and more.