Recombinant Active Mouse TNF Protein, His-tagged(C-ter)

Cat.No. : Tnf-308M
Product Overview : Recombinant Active Mouse TNF Protein with His tag (C-ter) was expressed in E. coli.
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Description : This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Source : E. coli
Species : Mouse
Tag : His(C-ter)
Form : Powder
Bio-activity : Determined by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is < 40 pg/ml. The specific activity of recombinant mouse TNF alpha is approximately > 2.5x 10^7 IU/mg.
AA Sequence : MLRSSSQNSSDKPVAHVVANHQVEE QLEWLSQRANALLANGMDLKDNQLV VPADGLYLVYSQVLFKGQGCPDYVL LTHTVSRFAISYQEKVNLLSAVKSP CPKDTPEGAELKPWYEPIYLGGVFQ LEKGDQLSAEVNLPKYLDFAESGQV YFGVIAL
Endotoxin : Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
Purity : > 98% (by SDS-PAGE)
Applications : SDS-PAGE
Notes : For laboratory research only, not for drug, diagnostic or other use.
Storage : Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer : PBS (pH 8.0)
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to ensure sufficient re-dissolved.
SDS-PAGE:
Gene Name : Tnf tumor necrosis factor [ Mus musculus ]
Official Symbol : Tnf
Synonyms : TNF; tumor necrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434;
Gene ID : 21926
mRNA Refseq : NM_013693
Protein Refseq : NP_038721

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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