Recombinant Bovine Phosphodiesterase 5A, cGMP-specific

• Cat.No.: PDE5A-640B
• Product Overview: Recombinant bovine PDE5A (phosphodiesterase 5A) expressed inSf9 cells, 95kDa/subunit; homodimer.

Specification

• Species: Bovine
• Source: Insect Cells
• Tag: Non
• Description: PDE5A is a member of the cyclic nucleotide phosphodiesterase family. PDE5A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5"-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. PDE5A regulates nitric oxide (NO)-induced relaxation and cGMP breakdown in smooth muscle cells in vas deferens. PDE5A expression predisposes mice to adverse left ventricular remodeling after myocardial infarction. Furthermore, increased myocardial PDE5A expression in patients with advanced cardiomyopathy can contribute to the development of heart failure.
• Source/Host: Sf9 cells.
• Application: Study of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening.
• Concentration: 0.4mg/ml.
• Purity Detail: Partially purified by ammonium sulfate precipitation, hydroxyapatite and size-exclusion chromatorgraphy.
• Specific Activity: 4µmol/min/mg.
• Formulation: Liquid. In 25mM TEA, pH7.5, containing 1mM EGTA, 5mM MgCl2 and 30% glycerol.
• Short Term Storage: -20°C.
• Long Term Storage: -80°C.
• Use/Stability: Do not store diluted preparations.
• Handling: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PDE5A phosphodiesterase 5A, cGMP-specific [ Bos taurus ]
• Synonyms: phosphodiesterase 5A, cGMP-specific; PDE5A; cGMP-specific 3",5"-cyclic phosphodiesterase; CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase
• Gene ID: 281972
• mRNA Refseq: NM_174417
• Protein Refseq: NP_776842
• UniProt ID: Q28156
• Chromosome Location: 6
• Pathway: Purine metabolism
• Function: 3",5"-cyclic-GMP phosphodiesterase activity; cyclic-nucleotide phosphodiesterase activity; cGMP binding; hydrolase activity; metal ion binding; nucleotide binding