PDE5A is a member of the cyclic nucleotide phosphodiesterase family. PDE5A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5"-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. PDE5A regulates nitric oxide (NO)-induced relaxation and cGMP breakdown in smooth muscle cells in vas deferens. PDE5A expression predisposes mice to adverse left ventricular remodeling after myocardial infarction. Furthermore, increased myocardial PDE5A expression in patients with advanced cardiomyopathy can contribute to the development of heart failure.
Source/Host :
Sf9 cells.
Application :
Study of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening.
Concentration :
0.4mg/ml.
Purity Detail :
Partially purified by ammonium sulfate precipitation, hydroxyapatite and size-exclusion chromatorgraphy.
Specific Activity :
4µmol/min/mg.
Formulation :
Liquid. In 25mM TEA, pH7.5, containing 1mM EGTA, 5mM MgCl2 and 30% glycerol.
Short Term Storage :
-20°C.
Long Term Storage :
-80°C.
Use/Stability :
Do not store diluted preparations.
Handling :
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.