Active Recombinant Canine EGF protein(Asn973-Arg1024)

• Cat.No.: EGF-4366C
• Product Overview: Recombinant Canine EGF (NP_001003094.1) (Asn973-Arg1024) was expressed in Yeast.

Specification

• Source: Yeast
• Species: Canine
• Tag: N/A
• Protein length: Asn973-Arg1024
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.
• Molecular Mass: The recombinant canine EGF consists of 52 amino acids and predicts a molecular mass of 6.2 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.